首页> 外国专利> Identification of toxin-binding protein involved in resistance to Cry1 toxins, and related screening methods

Identification of toxin-binding protein involved in resistance to Cry1 toxins, and related screening methods

机译：鉴定对 Cry1 毒素具有抗性的毒素结合蛋白及其筛选方法

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The subject invention relates in part to the surprising and unexpected discovery that insects that are resistant to Bacillus thuringiensis Cry toxins have measurably altered alkaline phosphatase (ALP) activity as compared to insects that are susceptible to Cry toxins. This and other surprising discoveries reported herein have broad implications in areas such as managing and monitoring the development of insect resistance to B.t. toxins. For example, the subject invention provides a simple and fast assay (enzymatic or otherwise) for detecting ALP activity levels and thereby monitoring the development of resistance by insects to crystal protein insect toxins. There was no prior motivation or suggestion to go about resistance monitoring using this simple and easy approach. 展开▼

机译：本发明部分涉及令人惊讶和出乎意料的发现，即与对易受Cry毒素感染的昆虫相比，对苏云金芽孢杆菌Cry毒素具有抗性的昆虫具有可测量地改变的碱性磷酸酶（ALP）活性。本文报道的这一发现和其他令人惊讶的发现，在诸如管理和监测昆虫对Btt的抗性发展方面具有广泛的意义。毒素。例如，本发明提供了用于检测ALP活性水平并由此监测昆虫对晶体蛋白昆虫毒素的抗性发展的简单且快速的测定法（酶法或其他方法）。没有事先动机或建议使用这种简单的方法进行电阻监测。展开▼

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公开/公告号US8802389B2

专利类型

公开/公告日2014-08-12

原文格式PDF

申请/专利权人 MICHAEL J. ADANG;REBECCA MCNALL;JUAN LUIS JURAT FUENTES;
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申请/专利号US201113274646

发明设计人 MICHAEL J. ADANG;REBECCA MCNALL;JUAN LUIS JURAT FUENTES;
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申请日2011-10-17

分类号A01N25/00;C12N1/20;C12Q1/00;C12Q1/02;C12Q1/42;G01N33/53;G01N33/554;G01N33/567;G01N33/569;

国家 US

入库时间 2022-08-21 16:07:01

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3. IDENTIFICATION OF TOXIN-BINDING PROTEIN INVOLVED IN RESISTANCE TO CRY1 TOXINS, AND RELATED SCREENING METHODS [P] . 美国专利： US2012034610A1 . 2012-02-09

机译：抗Cry1毒素的毒素结合蛋白的鉴定及筛选方法

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机译：与Cry1毒素抗性有关的毒素结合蛋白的鉴定及相关筛选方法

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机译：抗Cry1毒素的毒素结合蛋白的鉴定及筛选方法

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