首页> 外国专利> Arabinose isomerase expressed from Corynebacterium genus and tagatose manufacturing method by using it

Arabinose isomerase expressed from Corynebacterium genus and tagatose manufacturing method by using it

机译：棒状杆菌属表达的阿拉伯糖异构酶及其制备塔格糖的方法

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摘要

The present invention relates to a thermophilic arabinose isomerase and a method of manufacturing tagatose using the same, and more precisely, a gene encoding arabinose isomerase originating from the thermophile Thermotoga neapolitana DSM 5068, a recombinant expression vector containing the gene, a method of preparing a food grade thermophilic arabinose isomerase from the recombinant GRAS (Generally Recognized As Safe) strain transformed with the said expression vector, and a method of preparing tagatose from galactose using the said enzyme.

机译：本发明涉及嗜热的阿拉伯糖异构酶和使用其的塔格糖的制造方法，更准确地说，涉及来源于嗜热菌 Thermotoga neapolitana DSM 5068的编码阿拉伯糖异构酶的基因，该重组表达载体含有嗜热的阿拉伯糖。基因，从用所述表达载体转化的重组GRAS（通常公认安全）菌株制备食品级嗜热阿拉伯糖异构酶的方法，以及使用所述酶由半乳糖制备塔格糖的方法。 展开▼

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公开/公告号US8802393B2

专利类型

公开/公告日2014-08-12

原文格式PDF

申请/专利权人 SEONG-BO KIM;YOUNG-MI LEE;SEUNG-WON PARK;JUNG-HOON KIM;SANG-HOON SONG;KANG-PYO LEE;HYE-WON KIM;HYE-JIN CHOI;
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申请/专利号US20080253454

发明设计人 KANG-PYO LEE;JUNG-HOON KIM;HYE-WON KIM;SEONG-BO KIM;SANG-HOON SONG;YOUNG-MI LEE;SEUNG-WON PARK;HYE-JIN CHOI;
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申请日2008-10-17

分类号C12P21/06;C12P19/00;C12P19/20;C12N9/00;C12N9/92;C12N1/20;C12N15/00;C07H21/04;

国家 US

入库时间 2022-08-21 16:07:01

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1. 由棒状杆菌属表达的阿拉伯糖异构酶和使用其制造塔格糖的方法 [P] . 中国专利： CN101631856B . 2013.01.30

2. 由棒状杆菌属表达的阿拉伯糖异构酶和使用其制造塔格糖的方法 [P] . 中国专利： CN101631856A . 2010-01-20

3. ARABINOSE ISOMERASE EXPRESSED FROM CORYNEBACTERIUM GENUS AND TAGATOSE MANUFACTURING METHOD BY USING IT [P] . 欧洲知识产权局专利： EP2087108B1 . 2013-09-18

机译：棒状杆菌属表达的阿拉伯糖异构酶及其制备方法

4. ARABINOSE ISOMERASE EXPRESSED FROM CORYNEBACTERIUM GENUS AND TAGATOSE MANUFACTURING METHOD BY USING IT [P] . NZ576797A . 2011-06-30

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5. Arabinose isomerase expressed from corynebacterium genus and tagatose manufacturing method by using it [P] . AU2007326238B2 . 2011-06-30

机译：棒杆菌属表达的阿拉伯糖异构酶及其使用的塔格糖制备方法

1. Phosphoglucose isomerase gene expression as a prognostic biomarker of gastric cancer [J] . Han-Chen Huang, Xian-Zi Wen, Hua Xue, 中国癌症研究（英文版） . 2019,第005期

2. Cloning and Expression Analysis of Chalcone Synthase and Chalcone Isomerase Encoding Genes in Gossypium hirsutum [J] . 无农业生物技术：英文版 . 2018,第002期

3. Expression and Characterization of ArgR, An Arginine Regulatory Protein in Corynebacterium crenatum [J] . CHEN Xue Lan, ZHANG Bin, TANG Li, 生物医学与环境科学（英文版） . 2014,第006期

4. Co-expression of L-arabinose isomerase and D-glucose isomerase in E.coli and development of an efficient process producing simultaneously D-tagatose and D-fructose [J] . Moez Rhimi, Ezzedine Ben Messaoud, Mohamed Ali Borgi Enzyme and Microbial Technology . 2007,第6期

5. Two-stage biosynthesis of D-tagatose from milk whey powder by an engineered Escherichia coli strain expressing L-arabinose isomerase from Lactobacillus plantarum [J] . Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies . 2020,第期

6. Production of D: -tagatose at high temperatures using immobilized Escherichia coli cells expressing L: -arabinose isomerase from Thermotoga neapolitana [J] . Hong YH, Lee DW, Lee SJ, Biotechnology Letters . 2007,第4期

7. Biological Synthesis of D-tagatose Using L-arabinose Isomerase [C] . Xianghui Qi, Jingfei Zhu, Yan Luo International Conference on Molecular Imprinting . 2015

8. THE PHYSIOLOGY OF THE GENUS BACILLUS. A STUDY OF THE METABOLISM OF CERTAIN MEMBERS OF THE GENUS BACILLUS IN RELATION TO THEIR ABILITY TO FERMENT GLUCOSE, D-ARABINOSE AND L-ARABINOSE. [D] . BOZEMAN, SAMUEL RICHMOND. 1945

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10. Overexpression of L-Arabinose Isomerase for Production of the Low-Calorie Bulk Sweetener D-Tagatose [O] . Monika Van Holsbeeck 2014

1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004）（P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期

2. Gene Expression:A Review on Methods for the Study of Defense-Related Gene Differential Expression in Plants [J] . Alice Casassola ,Sandra Patussi Brammer ,Márcia Soares Chaves . 美国植物学期刊（英文） . 2013,第12期

3. L-阿拉伯糖异构酶及其在D-塔格糖生产中的应用 [C] . Zheng Xu ,徐铮 ,Hong Xu . 2010年全国淀粉糖、多元醇技术与发展研讨会 . 2010

4. 塔格糖的生物制备及L-阿拉伯糖异构酶性质研究 [A] . 张华 . 2007