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Device and method for improving sample injection and stacking
Device and method for improving sample injection and stacking
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机译:改善样品注入和堆叠的装置和方法
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摘要
The invention provides devices and methods for isolating one or more sample components of a sample material following separation of the sample material into a plurality of sample components. A device includes a separation channel having a sample loading well. A low-conductivity buffer is disposed in the loading well, the buffer having a conductivity0.2 mS/cm. In a method, a buffer is loaded into a loading well in fluid communication with a separation channel of a device. A sample material having a conductivity higher than that of the buffer is then loaded into the loading well such that the sample material is disposed beneath the buffer, the buffer disposed over and covering the sample material. The sample material is separated into a plurality of separated components in the separation channel, and a separated component is collected from a collection well disposed in a collection leg of the device.
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机译:本发明提供在将样品材料分离成多个样品组分之后,用于分离样品材料的一种或多种样品组分的装置和方法。装置包括具有样品加载孔的分离通道。将低电导率的缓冲液放置在装载井中,该缓冲液的电导率<0.2 mS / cm。在一种方法中,将缓冲液装载到与设备的分离通道流体连通的装载井中。然后将具有比缓冲剂的电导率高的电导率的样品材料装载到装载井中,使得样品材料被布置在缓冲剂下方,缓冲剂被布置在样品材料上方并覆盖样品材料。样品材料在分离通道中被分离成多个分离的组分,并且分离的组分从设置在装置的收集腿中的收集井收集。
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