Method for increasing the secreu00c7u00c3o a POI from a cu00c9lula eucariu00d3tica, use of a sequence of nucleotu00cddeo nucleotu00cddeo sequence, sequence, promoter of yeast expression vector eucariu00d3tico, and uses a vector of expression and a sequence promoter of yeast.

摘要

The present invention relates to be used for increasing the method for secreting protein of interest (POI) from eukaryotic cell, described method comprises the albumen coexpression that makes POI and at least a enhancing protein excretion, and described enhancing albumen is selected from the group of being made up of BMH2, BFR2, COG6, COY1, CUP5, IMH1, KIN2, SEC31, SSA4 and SSE1.The invention still further relates to the promoter sequence of the PET9 gene of yeast promoter sequence, particularly pichia pastoris, it has the promoter activity of increase under comparable conditions with respect to the proteic promoter sequence of GAP.The invention still further relates to the expression vector and the application of described expression vector in the expression of POI in host cell that comprise described promoter sequence.The invention still further relates to the Yeast bacterium promoter sequence from the gene of pichia pastoris, described Yeast bacterium promoter sequence can be used for the expression of POI in yeast.