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ABLE TO FERMENTATIVE BACTERIA ABLE TO MAINTAIN GLUCOSE, MANOSE AND XYLOSE SIMILARLY AND, PROCESS TO PRODUCE ETHANOL
ABLE TO FERMENTATIVE BACTERIA ABLE TO MAINTAIN GLUCOSE, MANOSE AND XYLOSE SIMILARLY AND, PROCESS TO PRODUCE ETHANOL
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机译:能够发酵的细菌能够类似地保持葡萄糖,甘露糖和木糖以及生产乙醇的过程
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摘要
The object is to develop a bacterium capable of fermenting glucose, mannose and xylose simultaneously, which can ferment a saccharified solution of a cellulose-type or lignocellulose-type biomass resource to produce ethanol, and to construct an energy-saving high-efficiency bioethanol conversion process. Thus, disclosed is Zymomonas mobilis bacterium which is prepared by integrating a gene encoding a phosphomannose isomerase derived from Escharichia coli into a levansucrase gene located on the chromosome by the double cross-over by means of a homologous recombsination method, and then introducing recombinant DNA prepared by binding a DNA fragment containing genes encoding a xylose isomerase, a xylulokinase, a transaldolase and a transketolase, respectively, all derived from Escherichia coli to a vector. Also disclosed is a method for producing ethanol by continuously fermenting a saccharified solution of a cellulose-type biomass resource in a system on which the Zymomonas mobilis bacterium is immobilized.
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