A method for culturing mammalian cells secreting heterogeneous protein in a cell culture supernatant and for culturing mammalian cells secreting factor VIII in a pot.

摘要

Culturing heterologous protein-secreting mammalian cells, such as CHO or BHK cells, at 35.1-36.5°C and/or at pH 7.15-7.20 and/or at a dissolved CO 2 concentration of 10% or lower. Preferred heterologous proteins are Factor VIII, ADAMTS-13, furin or Factor VII.