A NOVEL CELL LINE DEVELOPMENT STRATEGY TO PRODUCE RECOMBINAN PROTEINS USING TWIN VECTOR EXPRESSION SYSTEM

摘要

This invention relates to a process for developing a stable cell line for high level expression of recombinant proteins by co-transfection of two mammalian expression vectors wherein the gene for desired protein of interest (POI) along with signal sequence and reading frame are cloned/ inserted into two vectors independently, one having a DHFR expression cassette and the other having a selection marker other than DHFR.