NATURALLY DERIVED WATER SOLUBLE FRACTION OF ISPHAGULA MUCILAGE AND ITS USES THEREOF IN THE PREPARATION OF NANOPARTICULATED CARRIER FOR OCULAR DELIVERY

摘要

The water soluble fraction of mucilage from the seeds of isphagula, are yet to be explored for their use as natural polymers in the preparation of nanoparticlulate system especially for the ocular drug delivery. Therefore, in the present study, these water soluble fraction of mucilage were isolated and charcterized using X-ray diffraction spectrometry (XRD), Fourier transform infrared spectroscopy (FT-IR), Maldi/GC-MS, elemental analysis, ID (H and 13C) and 2D (HMQC, COSY) nuclear magnetic resonance spectroscopy (NMR). The characterterized mucilage was formulated in to nanoparticulate system using their complementarity charged polymer chitosan. These nanoreservoirs were evaluated for their physico-chemical and toxicity profile in ocular drug delivery using in vitro (HET-CAM and Haemolysis) and in vivo model on rabbit eyes. The isphagula mucilage was identified to be an arabinoxylan polysaccharide chain consisting of 4 units of arabinofuranose attached to the backbone of 9 xylopyrannose units in 1:3 ratio. The formed nanoparticles were found to be spherical with particle size ranging between 107.1 nm and zeta potential of 33.9 mV. The formulated nanocarrier were observed to be non-irritant and non-toxic upto 2000 �g/ml and therefore this mucilage can be the alternatives of anionic polymers for the ocular delivery system.