A TISSUE CULTURE MEDIUM AND A METHOD FOR PRODUCING BANANA PLANTS IN VITRO

摘要

The disclosure relates to a process of tissue culture for regeneration of Banana plants. The process consists of following steps: (a) cutting and chopping the banana suckers to obtain explants; (b) sterilizing the explants by treating with at least one sterilizing agent to obtain sterilized explants; (c) culturing the sterilized explants in a pre-sterilized first medium for shoot induction and multiplication to develop several proliferating shoots; (d) harvesting the developed shoots; (e) culturing the harvested shoots in pre-sterilized second medium for root induction and development to generate functional roots; obtaining the regenerated plantlets; and (f) transferring the regenerated plantlets to the soil for growing Banana plant. The disclosure also provides a culture medium, a First culture medium capable of efficient shoot induction and multiplication in explants from Banana plant and a second culture medium capable of root induction and development of rooting system.