IN VITRO CULTURING METHOD AND SYSTEM WITH INDICATOR OF NUTRIMENTAL PRESENCE USING SALTS OF LOW SATURATION POINT.

摘要

The in vitro culturing method and system with an indicator of nutrimental presence using salts of low saturation point, facilitates the detection of depletion of nutriments, having as an indicator a precipitate derived from the oversaturation of the medium through the use of salts with a low saturation point. The method comprises the sprout, growth and development of vascular plants in a sterile medium, which uses salts of low saturation point, salts of high solubility, an inert support, a sucrose source and a chelating agent at a pH of 5.6 +/- 0.2., with a photoperiod of 16/8 light/darkness hours, at a temperature of 25 +/- 2°C and 1800 lux; the system refers to a container, the culture medium with the indicator of presence of nutrients (medium IN), where the organism may be developed. The best concentration to promote, in a lower time, the sprout of Catasetum integerrimun, is IN 25% and IN 100% for phases of growth and development.