Toxicity analysis of infectious pancreatic necrosis virus in fish

摘要

It refers to a method to determine the serum of infectious pancreatic necrosis virus (IPNV) as SP in fish samples, which is characterized by: (a) determining whether IPNV exists in fish population; (b) extracting RNA from selected samples in stage a;(c) amplification, discrete region of VP2 protein, containing 271 and 221 residues; (d) purification of amplification products produced at level C;(E) sort the pure amplifier products of level (d);The obtained nucleoside sequence was transformed into amino acid sequence; (E) (f) the position of treonin or prolina residue in position 217 and alanina residue in position 221 in amino acid sequence obtained from stage E were determined to determine the toxicity of IPNV respectively.