Method for sterilization bulbs of Allium ursinum bear L garlic and obtaining shoots in in vitro cultures

摘要

the subject of the invention is a sterilizing onions garlic allium ursinum l and obtain shoots bear in cultures in vitro, the selection method of active substances and plant material sterilisation time treatment of them in order to obtain sterile culture eksplantatu00f3w in warunka ch in vitro.method of sterilizing onions garlic allium ursinum l and obtain shoots bear in cultures in vitro, is that of ex vitro conditions onions, clean water, cut off the roots, then subjected to an factors sterilisation - 96% ethyl alcohol and the burner flame. 30% hydrogen peroxide or uv radiation (40 '),then washed in distilled water and a standard medium ms (murashige and skoog murashige (1962)