Procedure for culturing cells by continuous perfusion and alternating tangential flow

摘要

Use of a perfusion cell culture procedure in which a cell culture comprising a cell culture medium and a cell suspension is circulated on a filter module comprising hollow fibers, in which the flow on the filter module it is an alternating tangential flow, for the reduction of the aggregation of the cells in the cell culture, in which these cells have the tendency to form easily or inherently aggregates during the culture, in which no more than 5% of the cells in The culture forms suspended aggregates of at least 5 cells.