PROCESS FOR CELL CULTURING BY CONTINUOUS PERFUSION AND ALTERNATING TANGENTIAL FLOW

摘要

PROBLEM TO BE SOLVED: To provide a process for culturing cells by continuous perfusion culturing of a cell culture comprising a cell culture medium and cells.SOLUTION: A perfusion culture included in a bioreactor comprises (a) a cell culture medium and (b) a suspension of animal cells that easily or inherently form aggregates during culturing, with a viable cell density of at least 80×106 suspension cells per mL culture medium, where the aggregates of at least 5 cells comprise at most 5% of the total amount of cells.SELECTED DRAWING: None