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PROCESS FOR CELL CULTURING BY CONTINUOUS PERFUSION AND ALTERNATING TANGENTIAL FLOW
PROCESS FOR CELL CULTURING BY CONTINUOUS PERFUSION AND ALTERNATING TANGENTIAL FLOW
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机译:连续灌注和交替切向流培养细胞的过程
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#$%^&*AU2013203461B220160317.pdf#####ABSTRACT A method for limiting cell aggregation during cell culture to a level wherein no more than 5% of the animal cells in suspension in the culture comprise aggregates of at least 5 cells, comprising: carrying out continuous perfusion culturing of a cell culture comprising cell culture medium and animal cells that easily or inherently form aggregates during culturing, wherein the continuous cell culturing comprises: (a) adding cell culture medium to the cell culture, and (b) circulating the cell culture through a filter module comprising hollow fibers, in an alternating tangential flow resulting in an outflow of cell culture liquid through the pores of the filter module having a lower animal cell density per ml than the cell culture prior to circulating through the filter module, wherein no more than 5% of the animal cells in the culture form aggregates in suspension of at least 5 cells during the continuous perfusion culturing, and wherein the continuous perfusion culturing is continued until animal cells are present in the cell culture at a density of at least 80 x 106 viable animal cells/ml.
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机译:#$%^&* AU2013203461B220160317.pdf #####抽象在细胞培养过程中将细胞聚集限制在不超过5%的水平的方法在培养物中悬浮的动物细胞中至少有5个细胞聚集在一起,包括:进行包含细胞培养基的细胞培养物的连续灌注培养和动物细胞在培养过程中容易或固有地形成聚集体,其中连续细胞培养包括:(a)在细胞培养物中加入细胞培养基,以及(b)使细胞培养物循环通过包含中空纤维的过滤器模块,交替的切向流导致细胞培养液通过细胞的孔流出过滤模块的每毫升动物细胞密度低于循环前的细胞培养物通过过滤器模块,其中培养物中不超过5%的动物细胞在在连续灌注培养过程中至少有5个细胞,其中持续进行灌注培养,直到动物细胞以至少80 x 106个活动物细胞/毫升。
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