NOVEL CENTROMERIC PROTEIN SHUGOSHIN

摘要

The present invention is to provide meiosis-specific novelkinetochore protein Sgol ( shugoshin) derived from fission yeastSchizosaccharomyces pombe, and a homologue or paralogue thereofhaving a regulatory activity of chromosome segregation; andDNAs encoding them; as a factor ensuring the retention ofunidirection and cohesion in sister centromere at meiosis I incooperation with cohesin. To elucidate the proteinsprotecting Rec8 during anaphase, the present inventor screenedin fission yeast genes for a gene that inhibits mitotic growthand prevents sister chromatid from the separation at anaphase,when co-expressed with Rec8 . In this approach,meiosis-specific protein Sgol that protects (Shugo)centromeric Rec8 from the degradation at anaphase I wasindentified. Further, a budding yeast Sgol homologue and afission yeast mitotic paralogue Sgo2 were identified.