Limonoids from the Fruits of Melia toosendan and Their NF-kB Modulating Activities

摘要

#$%^&*AU2014100777A420140814.pdf#####Limonoids from the Fruits of Melia toosendan and Their NF-xIB Modulating Activities ABSTRACT Meliaceous limonoids are well known bioactive components of natural pesticides and contemporary medicines. In this study, we identified eight new ring C-seco and ring intact limonoids, named meliatoosins A-H (compounds 1-8), along with 25 known limonoids (compounds 9-33) from the fruits of Melia toosendan (Meliaceae). Their structures were elucidated on the basis of extensive spectroscopic methods including HRMS, ID and 2D NMR experiments. 29 components were evaluated for NF-xIB activities by examining their effects on TNFu-stimulated NF-xIB luciferase activity in HepG2-NF-xB-Luc cells. Among them, 8 compounds (4, 6, 25, 26, 28-31) significantly enhanced the TNFu-induced NF-xIB luciferase activity at 10 ptM, while 10 compounds (11-13, 16, 19, 20, 22-24, 27) suppressed NF-xIB activation. These results suggested that limonoids from M. toosendan could biphasically regulate the TNFu-induced NF-xIB activation. DocRef#00126821-CX M006.030.DRF 26 spec final1/5 0j 23 22/0 100 j0_ /0 0 0 18 _ 21 0 119 11 30 1522 - - ::20 - 15 17 23 0 12- 1 2 1 8 14 20 HO 19 1130 13 17 HO 0 2 9 H1 10 9 14 15 29 2 3 5 6 'OH '" "OH R1 R2 29 28 '-d 1 A OAc 2 OH OAc 4 5 3 B OH R O O HOO HO HO 0/ , 0 A0] . OH 0'l 0 0 R2 R1 R2 0 6 OH C 8 7 OAc D A:4 B: O 0 C: O D: 4 2' 2 F 2' 2' Figure 1