CELL-BASED ACTIVATION OF DEVITALIZED ENGINEERED GRAFTS FOR TISSUE REPAIR

摘要

The invention relates to a method for production of a graft for use in a method of connective tissue repair or connective tissue regeneration or connective tissue replacement, characterized by (a) providing a hypertrophic cartilaginous template (HCT), (b) providing a preparation of osteoclast precursor cells, (c) seeding said preparation onto said HCT under conditions of cell culture in the presence of MCSF and RANKL, yielding seeded HCT, and (d) maintaining said seeded HCT under conditions of cell culture for a period of at least 12 hrs. The invention further relates to grafts provided by the method of the invention for repair, regeneration and replacement of connective tissue, particularly bone.