HUMANIZED MONOCLONAL ANTIBODY IN EXTRACELLULAR DOMAIN OF ANTI-HUMAN DEATH RECEPTOR 5

摘要

The present invention provides a humanized monoclonal antibody in an extracellular domain of an anti-human death receptor 5 (DR5), comprising a light chain variable region amino acid sequence which has at least 90% identity of an amino acid sequence shown by the SEQ ID NO:1, a heavy chain variable region amino acid sequence which has at least 90% identity of an amino acid sequence shown by the SEQ ID NO:2, and a constant region with a human antibody function IgG1. The present invention further provides a coding nucleotide sequence of the humanized monoclonal antibody, a recombination eukaryon expression vector, and a production method for the antibody, composition and use thereof. The humanized monoclonal antibody of the present invention has a specific apoptosis inducing activity on various tumor cells in vitro and vivo, may be used alone or used in combination with a natural ligand tumor necrosis factor related apoptosis inducing ligand of the DR5 or other drugs, and used for treatment of various tumors and diseases related to high expression of the DR5.