METHOD FOR DISTINGUISHING BETWEEN THE SPECIES COFFEA ARABICA AND COFFEA CANEPHORA AND HYBRIDS THEREOF BASED ON THE CONCOMITANT ANALYSIS OF NUCLEAR AND CHLOROPLASTIC DNA POLYMORPHISMS.

摘要

The present invention describes a method for distinguishing between Coffea arabica and Coffea canephora species and hybrids thereof, starting from plant materials of unknown origin such as coffee beans, based on polymorphisms of nuclear and chloroplast DNA. For this purpose polymorphic genetic markers have been identified and isolated, one of which is a portion of an Expressed Sequence Tag (EST), RM-00-O18, comprising or not comprising an insertion sequence and the other is a portion of plastid genome P-AC comprising a microsatellite. The use of polynucleotides comprising the two markers enables the two plant species of Coffea to be distinguished as well as related hybrids. The combined use of polynucleotides comprising the two markers enables the consistency between the nucleus and plastids to be established should the sample originate from Arabica/Robusta interspecies hybrids.