FIRST 180BP SEGMENT OF POLYHEDRIN GENE AND APPLICATION THEREOF

摘要

Provided are a first 180bp segment of a polyhedrin gene and an application thereof. The nucleotide sequence of the first 180bp segment of the polyhedrin gene is shown as SEQ ID NO:1. The segment is applicable to the expression of a fusion protein. Further provided is an expression carrier, which is formed by multiple cloning sites of a starting carrier being sequentially inserted with the foregoing segment and a protease digestion site segment. The sequence of the first 180bp of the polyhedrin gene in the present invention has more desirable properties than its full-length sequence, and can be connected to other target genes to express a fusion protein, which not only implements the effect of increasing an expression amount, but also increases the solubility of the fusion protein in a condition of the neutral pH value, so as to adapt to an optimal pH value for the function of protease digestion, and facilitate the acquisition of a target protein.