METHOD FOR PRODUCING RECOMBINANT HUMAN BASIC FIBROBLAST GROWTH FACTOR FROM RICE SEEDS

摘要

The present invention provides OsrbFGF genes optimized by genetic codon of rice, a related carrier and a method for preparing genetically modified rice seeds of the OsrbFGF and a method for separating and purifying the OsrbFGF. By using an expression carrier of an OsrbFGF specific expression of rice endosperm cells, the genetically modified rice seeds of the OsrbFGF are prepared, and the OsrbFGF is separated and purified from the rice seeds. The purity of the obtained OsrbFGF is 95%, and the OsrbFGF has the activity of promoting NIH/3T3 cell proliferation in vitro and promoting wound healing in vivo.