The present invention relates to the technical field of inspection and measurement. Disclosed is an enzymology detection method for detecting concentration of citrulline and asymmetric dimethylarginine (ADMA) in a reaction system by using an ATP detection method. The basic principle of the reaction is mainly as follows: ADMA is hydrolyzed by using dimethylarginine dimethylaminohydrolase to obtain citrulline; when ADP and Mg2+ exist, the citrulline is coupling-catalyzed by using ornithine carbamoyltransferase and carbamoyl phosphate kinase to finally obtain ornithine, carbon dioxide, ammonia gas, and ATP, wherein the released ATP can be detected by using a reagent. The present invention has the advantages of flexible and quick reaction, high accuracy, and low cost; ADMA or citrulline with the concentration of 0.1μmol/L at lowest can be detected, and the detection result approaches the concentration of ADMA in the normal serum and is far less than the concentration of citrulline in the normal serum, thereby providing a new applicable method for clinical ADMA detection.
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