IN-VITRO METHOD FOR MONOCLONAL ANTIBODY PRODUCTION USING NON-HUMAN ACT1 -DEFICIENT MICE

摘要

A method of producing an IgG type monoclonal antibody in vitro is described. The method includes the steps of: (A) providing B-cells obtained from a transgenic non-human mammal whose genome comprises a disruption of an Act1 gene; (B) contacting the B cells with an antigen under conditions which result in formation of an antibody specific to the antigen by the B cells; (C) fusing the B cells with one or more immortal cells, to produce one or more hybridomas which express IgG type monoclonal antibodies that specifically bind to the antigen; (D) selecting a hybridoma which expresses an antibody specific to the antigen; and (E) obtaining monoclonal antibody produced by the selected hybridoma. Kits for carrying out the method of producing an IgG type monoclonal antibody are also described.