首页> 外国专利> NOVEL GENE FOR BACILLUS THURINGIENSIS ENDOTOXIN PROTEIN HAVING ENHANCED INSECTICIDAL ACTIVITY AGAINST LEPIDOPTERAN PESTS, AND METHOD FOR PRODUCING SAME

NOVEL GENE FOR BACILLUS THURINGIENSIS ENDOTOXIN PROTEIN HAVING ENHANCED INSECTICIDAL ACTIVITY AGAINST LEPIDOPTERAN PESTS, AND METHOD FOR PRODUCING SAME

机译：苏云金芽孢杆菌内毒素毒素蛋白的新型基因，具有增强的对抗鳞翅目害虫的杀虫活性，及其生产方法

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摘要

The present invention relates to a novel gene for Bacillus thuringiensis endotoxin protein having enhanced insecticidal activity against lepidopteran pests, such as Plutella xylostella, Spodoptera exigua, and Ostrinia furnacalis, and a method for producing the same. The present invention provides a novel gene for recombinant endotoxin protein in which an amino acid residue sequence associated with a host range of cry1-type endotoxin protein having insecticidal activity against lepidopteran pests is substituted through multi-site-directed mutagenesis so as to produce insect-resistant transgenic crops, and a method for producing the same.

机译：本发明涉及一种新的苏云金芽孢杆菌内毒素蛋白基因，该蛋白具有增强的对鳞翅目害虫如小菜蛾，小菜夜蛾等鳞翅目害虫的杀虫活性。和 Ostrinia furnacalis ，以及生产它的方法。本发明提供了一种用于重组内毒素蛋白的新基因，其中与具有对鳞翅目害虫具有杀虫活性的 cry1 型内毒素蛋白的宿主范围相关的氨基酸残基序列通过多部位定向被取代。诱变以产生抗虫转基因农作物及其生产方法。 展开▼

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公开/公告号WO2014157820A1

专利类型

公开/公告日2014-10-02

原文格式PDF

申请/专利权人 SNU R&DB FOUNDATION;
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申请/专利号WO2014KR00657

发明设计人 JE YEON HO;CHOI JAE YOUNG;KIM SONG EUN;KIM JAE SU;
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申请日2014-01-23

分类号C12N15/32;C07K14/325;A01N63/02;

国家 WO

入库时间 2022-08-21 15:46:50

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专利

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1. 对鳞翅目害虫高效的苏云金芽孢杆菌菌株及其杀虫基因和应用 [P] . 中国专利： CN101074427A . 2007-11-21

2. 对鳞翅目昆虫有活性的苏云金芽孢杆菌δ内毒素组合物及其使用方法 [P] . 中国专利： CN1390259A . 2003-01-08

3. Crylca5 isolated a Protein from Bacillus thuringiensis, a n-terminal Fragment of the Protein, a synthetic Gene that encodes the Protein.Unrocio insecticide and a method to protect Crop plants from a lepidopteran Insect Pest [P] . AR008885A1 . 2000-02-23

机译： Crylca5从苏云金芽胞杆菌中分离出一种蛋白质，该蛋白质的n端片段，一种编码该蛋白质的合成基因，非杀虫剂和一种保护植物免受鳞翅目昆虫危害的方法

4. Plasmid containing DNA from a PLASMIDFRAKTION OF B.THURINGIENSIS, E.COLI bacteria strain containing the plasmid, E.COLI genetically engineered bacterial strain, method for preparing the plasmid method of creating a genetically engineered bacterial strain E.COLI, process for producing Bacillus thuringiensis CRYSTAL PROTEINS AND PROCEDURE TO OBTAIN insecticidal activity IN AN ENVIRONMENT [P] . DK160998B . 1991-05-13

机译：包含来自苏云金芽孢杆菌的质粒的DNA的质粒，包含该质粒的大肠杆菌大肠杆菌菌株，大肠杆菌基因工程菌菌株，制备该质粒的方法，用于制备遗传细菌菌株大肠杆菌的方法，生产苏云金芽孢杆菌的方法在环境中的晶体蛋白和获得杀虫活性的方法

5. Novel Bacillus thuringiensis gene encoding crystal protein having improved insecticidal activity against lepidopteran insects and preparing method thereof [P] . 韩国专利： KR101555124B1 . 2015-09-22

机译：苏云金芽孢杆菌新型编码晶体蛋白对鳞翅目昆虫有杀虫活性的基因及其制备方法

1. A Novel Bacillus thuringiensis Cry57 Protein Domain Swap Influence on Insecticidal Activity [J] . Shi Chen-chen, Zhou Guo-wang, Liu Zni-yang, 东北农业大学学报（英文版） . 2018,第003期

2. Reduced square Bacillus thuringiensis insecticidal protein content of transgenic cotton under N deficit [J] . CHEN Yuan, LIU Zhen-yu, Leila I.M.TAMBEL, 农业科学学报（英文版） . 2021,第001期

3. Reduced square Bacillus thuringiensis insecticidal protein content of transgenic cotton under N deficit [J] . CHEN Yuan, LIU Zhen-yu, Leila I.M.TAMBEL, 农业科学学报：英文版 . 2021,第001期

4. Comparative Analysis of Bacillus thuringiensis Vip3Aa57 and Vip3Aa62 Insecticidal Activities [J] . Shen Ya-wen, Li Shuai, Wang Jing, 东北农业大学学报（英文版） . 2021,第002期

5. Larvicidal activities of chinaberry, neem and Bacillus thuringiensis israelensis (Bti) to an insecticide resistant population of Anopheles arabiensis from Tolay, Southwest Ethiopia [J] . Assalif Demissew, Meshesha Balkew, Melaku Girma 亚太热带生物医学杂志（英文版） . 2016,第007期

6. Bacillus thuringiensis chimeric proteins Cry1A.2 and Cry1B.2 to control soybean lepidopteran pests: New domain combinations enhance insecticidal spectrum of activity and novel receptor contributions [J] . Danqi Chen, William J. Moar, Agoston Jerga, PLoS One . 2021,第6期

7. Insecticidal activity, putative binding proteins and histopathological effects of & IT;Bacillus thuringiensis & IT; Vip3(459) toxin on the lepidopteran pest & IT;Ectomyelois ceratoniae & IT; [J] . Boukedi Hanen, Tounsi Slim, Abdelkefi-Mesrati Lobna Acta tropica: Journal of Biomedical Sciences . 2018,第期

8. Activity of vegetative insecticidal proteins Vip3Aa58 and Vip3Aa59 of Bacillus thuringiensis against lepidopteran pests [J] . Baranek Jakub, Kaznowski Adam, Konecka Edyta, Journal of Invertebrate Pathology . 2015,第Null期

9. Hybrid Bacillus thuringiensis delta-endotoxins provide enhanced spectrum of activity against lepidopteran pests of cotton [C] . Saku Sivasupramaniam, Victor Kabuye, Tom Malvar, Beltwide Cotton Conferences . 2002

10. Investigation of the Bacillus thuringiensis insecticidal Cry1A toxin binding mechanism to lepidopteran midgut receptors. [D] . Jenkins, Jeremy Lee. 2000

11. Insecticidal Activity of Bacillus thuringiensis Cry1Bh1 against Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae) and Other Lepidopteran Pests [O] . Justin Lira, Jeff Beringer, Stephanie Burton, 2013

12. Culture medium of Bacillus thuringiensis serovar japonensis Buibui enhances the insecticidal activities of .DELTA.-endotoxins from B. thuringiensis serovar kurstaki and aizawai against lepidopterous pest insects. [O] . Shoji Asano, Katsutoshi Ogiwara, Makoto Takahashi, 2000

13. EPA Pesticide Fact Sheet: 'Bacillus thuringiensis' Cry3Bbl Protein and the Genetic Material Necessary for its Production (Vector ZMIR13L) in Event MON863 Corn and 'Bacillus thuringiensis' Cry1Ab Delta-Endotoxin and the Genetic Material Necessary for its [R] . 2005

1. 苏云金芽孢杆菌新型杀虫蛋白基因的研究与开发 [C] . 黄大防 ,中国农业科学院植物保护研究所 ,张杰 . 中国植物保护学会第九届会员代表大会暨2005年学术年会 . 2005

2. 苏云金芽孢杆菌营养期杀虫蛋白（Vip3）突变体的杀虫活性及其对胰蛋白酶的敏感性 [A] . 徐宁 . 2007