首页>
外国专利>
MONOMER ARCHITECTURE OF TAL NUCLEASE OR ZINC FINGER NUCLEASE FOR DNA MODIFICATION
MONOMER ARCHITECTURE OF TAL NUCLEASE OR ZINC FINGER NUCLEASE FOR DNA MODIFICATION
展开▼
机译:用于DNA修饰的tal核酸酶或锌指核酸酶的单体结构
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention provides compositions and methods for targeted cleavage of cellular chromatin in a region of interest and/or homologous recombination at a predetermined site in cells. Compositions include fusion polypeptides comprising a TAL effector binding or a zinc finger domain and an I-TevI homing endonuclease cleavage domain as well as nucleic acid sequence encoding the same. The use of the I-TevI domain allows for monomer endonuclease sequences to achieve cleavage of cellular chromatin and represents an advantage over prior endonucleases which require self-dimerization, and two nucleases with appropriate spacers.
展开▼