A METHOD FOR PRODUCING ORGANIC ACID BY FED-BATCH FEEDING OF CARBON SOURCE SUBSTRATE AND BASE

摘要

The present invention provides a fed-batch culture method comprising a step for feeding a carbon source and base by fed-batch culture to maintain the optimum pH for the growth of microorganism and a method for producing organic acid using the fed-batch culture method. According to the present invention, the optimum pH for survival of the organism to ferment the carbon source is maintained and the injection speed of the carbon source substrate, a base material, is adjusted accordingly at the same time by fed-batch feeding of a neutralizing agent such as ammonium bicarbonate or weak base containing alkali metal and a carbon source substrate when producing the organic acid by microorganism fermentation,thereby increasing the productivity, yield, and production intensity of the organic acid and improving the reliability and simplicity of fermentation process operation since the base and the carbon source are automatically injected depending on pH change. [Reference numerals] (AA) Top;(BB) Bottom;(CC) Empty space;(DD) Porous frit(top);(EE) Polymer;(FF) Porous frit(bottom);(LL) dipping;(MM) Steaming;(NN) Drying;(OO) Packaging