Novel gene from tidal flat metagenome and a novel protein displaying both phospholipase and lipase activities

摘要

The present invention exhibit phospholipase and lipase obtained simultaneously active meta-genome derived from tidal flats, and this new gene relates to a protein, specifically, a protein having all of the new gene and is coded from this phospholipase and lipase activity isolated from a genomic library of the tidal meth soil microorganism is expressed in a soluble form, so mass production is possible, and, Ni-NTA The protein obtained in remarkably high purity from, only one step of purification using a column, shows excellent activity at pH 5 to 10, 3 to maintain an excellent low-temperature activity and stability up to 40 , high resistance to various organic solvents Because of the, maintenance of the tablets and the conversion precision biomedical and chemical fields may be useful in a variety of industrial use and the like. ;