Methods for simultaneously determining Blood Coagulation Factor XIII and Transglutaminase 2 activities

摘要

The present invention is a blood clotting factor fibrinogen in a sample using an array 13 (FXIII) and trans posts capable of measuring the activity of the trans-amino Nation rutile MANIJEH 2 (TG2) at the same time the on-chip measurement system activity and can be used effectively with a blood coagulation factor 13 and transglutaminase 2-specific activity measured in the measuring system relates to a reaction composition that is optimized for. The present inventors buffer, pH, the measurement conditions were optimized as dithiothreitol and 5 (biotin amido) concentration of pentylamine and was produced by expression in a sample to determine the specific activity FXIII and TG2. The inventors have successfully applied to a measurement system for ball four 12-myristate 13-acetate was FXIII and TG2, and monitoring a change in the activity in THP-1 monocytic cells differentiated by interleukin-4. This assay is sensitive because of the active and fit for fast mass determination of FXIII and TG2 activity in cell signaling and cardiovascular pathophysiology research has a strong potential to investigate the discriminatory features of these isozyme. ;