COMPOSITION FOR DETECTING HEPATITIS C VIRUS(HCV) GENOTYPES COMPRISING PARALLEL BINDING STRUCTURED PNA PROBE SYSTEM AND DETECTION METHOD USING THEREOF

摘要

The present invention relates to a composition for simultaneously detecting both type 1 and type 2 HCV genes based on a double hybridization peptide nucleic acid (PNA) probe system in a parallel binding structure, and a method for simultaneously detecting type 1 and type 2 HCV genes using the composition and a real-time polymerase chain reaction. Upon executing the real-time polymerase chain reaction using the composition comprising the double hybridization PNA probe system in parallel binding structure of the present invention, type 1 and type 2 HCV genes in a tube can be accurately detected at the same time by excellent sensitivity, thereby being capable of a more prompt and accurate clinical diagnosis. [Reference numerals] (AA,KK) First probe; (BB,LL) Second probe; (CC,EE,MM,OO) N' (or C'); (DD,FF,NN,PP) C' (or N'); (GG,QQ) Target nucleotide; (HH,RR) Reporter (fluorescent material); (II,SS) Quencher (extinction material); (JJ,TT) Reporter or quencher