MICROSATELLITE MARKER FOR INDIVIDUALIZATION IN KOREAN NATIVE CHICKEN AND INDIVIDUALIZING METHOD USING THE SAME

摘要

The present invention relates to a method for individually identifying Korean native chickens using a microsatellite marker, and more specifically, provides a method for individually identifying Korean native chickens, the method comprising: a step of performing PCR amplification using microsatellite markers obtained by combining one or more sets of a first set consisting of microsatellite markers LEI0251, ADL0259, ADL0021, MCW0104, MCW0261, MCW0087, ADL0292, GCT0016, MCW0029, MCW0145, LEI0107, and ADL0304, and a second set consisting of microsatellite markers LEI0251, ADL0259, ADL0021, MCW0104, MCW0261, MCW0087, ADL0292, GCT0016, MCW0029, MCW0145, LEI0107, ADL0304, ADL0324, MCW0063, and MCW0264; and determining the genotype by performing electrophoresis on the product amplified in the step of performing PCR amplification to thereby detect alleles and analyze sizes thereof. The method according to the present invention enables individual identification and paternity identification in a more accurate manner than the morphological classification, in terms of identification of Korean native chickens, by using the microsatellite markers having high individual identification. Therefore, the method of the present invention is applied to the individual identification of Korean native chickens to produce positive effects in the preservation of Korean native chickens and reliability thereof, thereby preventing illegal distribution and contributing to the development of native chicken industry and an increase in the farm income.