Microsatellite Marker for individualization in Korean native chicken and individualizing Method Using the Same

摘要

The present invention relates to a method for identifying an individual using a microsatellite marker, and more particularly, to a method for identifying an individual of a traditional chicken using microsatellite markers, , A first set of ADL0304, a second set of LEI0251, ADL0259, ADL0021, MCW0104, MCW0261, MCW0087, ADL0292, GCT0016, MCW0029, MCW0145, LEI0107, ADL0304, ADL0324, MCW0063, MCW0264. A step of performing PCR amplification using a light marker, and a step of detecting an allele using an electrophoresis apparatus and analyzing the size of the product amplified in the amplifying step to determine a genotype. Since the method according to the present invention uses a microsatellite marker having high individuality, it is possible to identify the individual of the native chicken and the parent of the native chicken more precisely than the morphological classification standard. Therefore, the method of the present invention can be applied to the identification of the native chickens to control the illegal circulation by positively influencing the preservation and reliability of the native chickens, thereby contributing to the development of the traditional chicken industry and the income increase of the farm household.