METHOD FOR DETECTING AND DISTINGUISHING HOST-SPECIFIC STRAIN OF TOMATO YELLOW LEAF CURL VIRUS USING POLYMERASE CHAIN REACTION AND RESTRICTION FRAGMENT LENGTH POLYMORPHISM

摘要

The present invention relates to a method for detecting and distinguishing a host-specific strain of a Tomato yellow leaf curl virus (TYLCV) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). More specifically, the present invention relates to a method for detecting and distinguishing Korea, Israel, Spain, USA, Jordan 1, Jordan 2, and Jordan 3 which are seven strains of the Tomato yellow leaf curl virus (TYLCV) from an amplified product, which is amplified using a primer set for PCR for detecting a host-specific strain of a TYLCV, using iDde/iI, iFau/iI, oriBss/iSI which is a restriction enzyme. By means of the method according to the present invention, the infection of the TYLCV and the strain can be effectively confirmed within a short period of time without high-priced technical equipment such as sequence analysis equipment. Accordingly, the spread of the TYLCV is domestically prevented, and the inflow of domestically non-occurred strains can be prevented in a plant quarantine step, so that a huge economical loss can be remarkably reduced.;COPYRIGHT KIPO 2014