The present invention relates to a method for separating ginsenoside from a ginseng extract in one step using a counter-current chromatography. A certain amount and more of high purity of 14 kinds of ginsenoside can be obtained within a short time (100-400 minutes) through one chromatography process without going through a special process. Moreover, an expensive column with a limited reuse is not needed, and the use of a solvent is drastically reduced.
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