PURPOSE: Kazachstania exigua CCSY27 strain is provided to make makkoli (Korean rice wine) and to have probiotic properties, thereby manufacturing high quality makkoli by being used instead of Saccharomyces cerevisiae. CONSTITUTION: Kazachstania exigua CCSY27 (KACC93119P) strain is used for manufacturing makkoli. The makkoli is manufactured using grains as an ingredient and the strain. The grains contain rice and wheat mixed in a weight ratio of 5:5. [Reference numerals] (AA) Prepare rice; (BB) Prepare wheat(husk-removed wheat); (CC) Wash; (DD) Prepare rice flour; (EE) Prepare wheat flour; (FF) Dipping in water(8-12 hours, room temperature); (GG) Dipping in water(3-6 hours, room temperature); (HH) Kneading; (II,JJ) Steaming(30-60 minutes, 100C); (KK) Cool; (LL) Cool and cut; (MM) Culture spawn; (NN) Starch ingredient(rice:wheat=10:0, 8:2, 6:4, 5:5, 4:6, 2:8, 0:10); (OO) First mashing(yeast:nuruk:purified water); (PP) Second mashing(nuruk:starch:ingredient:purified water); (QQ) Ferment(25C, 7 days); (RR) Filter; (SS) Makkoli(Korean rice wine)
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