Development of recombinant Escherichia coli which can overflow fatty acid biosynthesis pathway by overproducing malonyl-CoA

摘要

The invention The present invention is transformed or co-transformed with an expression vector comprising the following nucleotide sequence The (cotransformed) carbonyl -CoA words provides for over-expression of E. coli: (a) acetyl -CoA carboxylic cyclase carboxyl transferase subunit alpha (acetyl-CoA carboxylase carboxytransferase subunit alpha) nucleotides encoding the sequence, (b) acetyl -CoA-carboxylic cyclase biotin carboxyl carrier protein (biotin carboxyl carrier protein) a nucleotide sequence encoding, (c) acetyl -CoA carboxylic cyclase biotin carboxyl cyclase subunit (biotin carboxylase subunit) a nucleotide sequence, and (d encoding ) acyl-carrier-protein S- malonyl transferase (acyl-carrier-protein S-malonyltransferase) at least one or more kinds of nucleotide sequence selected from the group consisting of New cluster ot draw encoding. The present invention provides an effect that can increase the content of fatty acids, as well as to be in and out of the cell itself, promote the production of acid words seconds Step material two kinds of fatty acid biosynthesis by a recombinant Escherichia coli. ;