首页> 外国专利> Vector for Mass Producing of Recombinant Protein Using Barley Ribosome-inactivating Protein and Method for Mass Producing of Protein Using the Same

Vector for Mass Producing of Recombinant Protein Using Barley Ribosome-inactivating Protein and Method for Mass Producing of Protein Using the Same

机译:大麦核糖体失活蛋白用于大规模生产重组蛋白的载体和使用该载体大规模生产蛋白的方法

摘要

The present invention using a ribosomal inhibiting protein (bRIP1) present in barley , the recombinant protein in microbial relates to a process for production of a water-soluble (soluble) state , more specifically, the desired protein and ribosome- inhibiting protein (bRIP1) a method for producing a protein using a recombinant expression vector and the vector such that the fusion protein expressed in a form of a water-soluble state relates to . According to the invention , than conventional recombinant protein was used to screen for receiving production of the protein, the recombinant protein can be mass-produced with high efficiency in prokaryotic cells with high efficiency of a water-soluble state , purification and separation of the produced recombinant protein also readily it is . ;
机译:本发明使用大麦中存在的核糖体抑制蛋白(bRIP1),微生物中的重组蛋白涉及产生水溶性(可溶性)状态的方法,更具体地,涉及所需蛋白和核糖体抑制蛋白(bRIP1)一种使用重组表达载体生产蛋白质的方法,该载体涉及以水溶性状态形式表达的融合蛋白。根据本发明,与使用常规重组蛋白来筛选该蛋白的生产相比,该重组蛋白可以在原核细胞中高效地大量生产,并且水溶性状态的效率高,纯化和分离所产生的蛋白。重组蛋白也很容易。 ;

著录项

  • 公开/公告号KR101423713B1

    专利类型

  • 公开/公告日2014-07-31

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20120085362

  • 发明设计人 김민경;송현규;이병길;

    申请日2012-08-03

  • 分类号C12N15/62;C12N15/63;C12N15/29;

  • 国家 KR

  • 入库时间 2022-08-21 15:40:26

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