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Method for Preparing Multi-functional Biomolecular Conjugates Using Two Kinds of Particle and Multi-functional Biomolecular Conjugates Prepared Therefrom
Method for Preparing Multi-functional Biomolecular Conjugates Using Two Kinds of Particle and Multi-functional Biomolecular Conjugates Prepared Therefrom
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机译:用两种颗粒和由此制备的多功能生物分子缀合物制备多功能生物分子缀合物的方法
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摘要
Disclosed herein are a method for manufacturing a multi-functional bio-material conjugate used as a biosensor for detecting microorganisms, and the like, and a multi-functional bio-material conjugate manufactured by means of the same. The method for manufacturing a multi-functional bio-material conjugate includes: (a) coating a first nanoparticle having magnetic or fluorescent characteristics with protein; (b) manufacturing a conjugate by adsorbing a second nanoparticle having metallic characteristics onto the first nanoparticle coated with protein; and (c) manufacturing the multi-functional bio-material conjugate by adsorbing a bio-material onto the conjugate. The method for manufacturing a multi-functional bio-material conjugate according to the present invention may prevent precipitation of the nanoparticles, easily immobilize the bio-material, and manufacture a bio-material conjugate having multiple functions, by using two kinds of the particles. In addition, the multi-functional bio-material conjugate manufactured by the present method may be used to detect microorganisms at up to a concentration of 101 cfu.
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