METHOD FOR PREPARING MULTI-FUNCTIONAL BIOMOLECULAR CONJUGATES USING TWO KINDS OF PARTICLE AND MULTI-FUNCTIONAL BIOMOLECULAR CONJUGATES PREPARED THEREFROM

摘要

The present invention relates to a method for manufacturing a multi-functional bio-material conjugate, and a multi-functional bio-material conjugate manufactured by means of same, and more specifically, to a method for manufacturing a multi-functional bio-material conjugate used as a biosensor for detecting microorganisms or similar, and a multi-functional bio-material conjugate manufactured by means of same. The method for manufacturing a multi-functional bio-material conjugate comprises: (a) a step for coating protein on a first nano particle having magnetic or fluorescent characteristics; (b) a step for manufacturing a conjugate by adhering a second nano particle having metallic characteristics on the first nano particle, coated with protein; and (c) a step for manufacturing a multi-functional bio-material conjugate by adhering bio-material to the conjugate. By using two kinds of particle, the method for manufacturing a multi-functional bio-material conjugate according to the present invention prevents the precipitation of nano particles, enables bio-material to be easily fixed, and enables the manufacture of a bio-material conjugate having multiple functions. In addition, the multi-functional bio-material conjugate manufactured via said method can be used to detect microorganisms, up to a concentration of 10¹ cfu.