首页> 外国专利> Primer composition for loop-mediated isothermal amplification reaction for detecting Turnip Yellow Mosaic virus, and use thereof

Primer composition for loop-mediated isothermal amplification reaction for detecting Turnip Yellow Mosaic virus, and use thereof

机译:用于检测萝卜黄花叶病毒的环介导等温扩增反应的引物组合物及其用途

摘要

The present invention relates to a set of four primers for isothermal amplification reaction, a composition including the same, and a method for detecting Turnip yellow mosaic virus using the composition. The detection method according to the present invention can effectively detect all six mutants of the Turnip yellow mosaic virus without a professional device in a short time using the isothermal amplification method. In addition, the Turnip yellow mosaic virus can be rapidly diagnose with a naked eye under natural light using highly concentrated SYBR Green I. Therefore, the present invention facilitates early detection of virus which harms farms cultivating Brassicaceae crops such as cabbages and the like, thereby making it possible to rapidly and efficiently construct a system diagnosing the Turnip yellow mosaic virus.
机译:本发明涉及用于等温扩增反应的一组四个引物,包括该引物的组合物以及使用该组合物检测芜菁黄色花叶病毒的方法。根据本发明的检测方法可以使用等温扩增方法在短时间内有效地检测芜菁黄花叶病毒的所有六个突变体而无需专业设备。另外,可以使用高浓度的SYBR Green I在自然光下用肉眼快速诊断萝卜黄色花叶病毒。因此,本发明有助于早期发现病毒,该病毒危害种植十字花科作物如卷心菜等的农场,从而可以快速有效地构建诊断芜菁黄色花叶病毒的系统。

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