NEW TEST SYSTEM E. colI / gsk3β / aphVIII FOR SCREENING OF INHIBITORS OF PROTEINKINASE gsk3β MAN

摘要

1. The E.coli / gsk3β / aphVIII test system is characterized in that it is a genetic construct based on the pET32a plasmid: the cloned genes aphVIII and gsk3β are under the same promoter, but have their own enhancers, ribosome binding sites (RBS), codons and terminators. The invention includes a set of pairs of oligonucleotides for cloning and site-directed mutagenesis. The E. coli / gsk3β / aphVIII test system allows selective screening of gsk3β protein kinase inhibitors.