METHOD OF DETECTION OF THE ADHESIVE ACTIVITY OF STRAINS OF YEASTLIKE GRIBOV CANDIDA ARE ALBICANS

摘要

A method for determining strains adhesive activity of yeasts Candida albicans, consists in the fact that the two fungi are prepared overnight culture 3 ml of the working cell suspension in phosphate buffer pH 5.6 ± 0.1 to 0.5 accurately standard McFarland turbidity, absorbance measurement is carried out working cell suspension was prepared in parallel with this nitrocellulose film from the immobilized hemoglobin, wherein for the preparation to collodion (cellulose nitrate) in an amount of 0.06 g was added 1.25 ml of butyl acetate, 0.75 ml toluene and 0.06 ml glutarovog aldehyde and 1-3 drops of hexane, the whole was stirred and the resulting solution was poured into a petri dish and dried under a fan to form a dry film, a nitrocellulose film was placed in a hemoglobin solution prepared from 0.05 g of hemoglobin dissolved in 5 ml of phosphate buffer solution with a pH 7, and incubated for 2 hours at room temperature, the resulting film was dried, the nitrocellulose film immobilized hemoglobin smpomeschayut area 7 into a tube with a working cell suspension after the preliminary measuring its optical density STI and incubated for 120 minutes at a temperature of 35 ° C, shaking several times a tube to obtain a homogeneous suspension of cells is again measured optical density of the cell suspension, the number of adherent cells per hemoglobin immobilized on the nitrocellulose film is determined by the difference of the initial and final optical density cell suspension.