METHOD FOR PRODUCING preparative antigens phloem-Limiting viruses

摘要

1. A process for preparing preparativyh amounts of viral antigen - a protein potato leaf roll virus (PLRV, PLRV) composed of chimeric viral particles, virions simulating PLRV, comprising the following steps: a) creating a recombinant DNA containing virus cDNA bleaching turnip vein (VPZHT, TVCV) belonging to tobamovirusam where VPZHT cDNA is under control of a plant promoter, at its end there is a transcription terminator, and the coat protein gene replaced by the gene VPZHT major proteins PLRV shell b) creating agrobakterialnog vector (plasmid) pCambia 1300 capable incorporated into the genome of the host plant and induce systemic infection; c) breeding the chimeric virus consisting of viral RNA in the host plant Nicotiana benthamian; d) recovering the chimeric virus from infected leaves of a plant-hozyaina.2 . A method for producing antisera to capsid protein of PLRV, comprising immunizing animals with viral particles produced by the process of claim 1.