METHOD OF CONDUCTING BY ALLELE- SPECIFIC PCR FOR genetic typing OF LARGE LIVESTOCK ON ALLELES THE B OF GENE CSN3

摘要

A method of allele-specific PCR for genotyping cattle with respect to alleles A and B CSN3 gene comprising preparing a nucleic acid sample, said sample introduction into the reaction mixture for PCR consisting of dNTPs, buffer system, Taq DNA polymerase, 4 allele specific primers; PCR; detecting the amplified DNA fragments by gel electrophoresis, characterized in that the other primer sequences are used that have additional non-complementary to any of the alleles CSN3 «mismatch-nucleotides" in position - 2 from the 3'-terminal nucleotide primers: AFd: 5'- GGGGGCTGTTCACACACAAAAACAGTAGAG-3 '(SEQ ID NO: 1), ARd: 5'-GGGGGGTGCCTAACCTTATACAGCCTTCCG-3' (SEQ ID NO: 2), BFd: 5'-CCCCCGTGAGCCTACAAGTACACCTACGAT-3 '(SEQ ID NO: 3), BRd: 5' -CCCCCGATGTCTCCTTAGAGTATTTAGCCC-3 '(SEQ ID NO: 4).