NEW HYDROGENASES EXTRACTED FROM THERMOCOCCUS SPP, GENES CODING THOSE HYDROGENASES, AND METHODS FOR HYDROGEN PRODUCTION USING MICROORGANISMS CONTAINING ABOVE SAID GENES

摘要

FIELD: biotechnologies.;SUBSTANCE: proposed ferments are as follows: hydrogenase and dehydrogenase extracted from Thermococcus onnurineus NA1 and having the sequences specified in the description, as well as their coding genes. A vector containing the above genes combined into FDH2-MFH2-MNH2 cluster is described. A host cell containing the above vector is described. Hydrogen production methods involving the following stages are proposed: preparation of medium in a cultivation vessel; supply of formiate to gaseous phase present in the cultivation vessel; cultivation of recombinant host cell or strain T. onnurineus in the cultivation vessel; extraction of hydrogen from the cultivation vessel.;EFFECT: invention allows more effective production of hydrogen by means of a microbiological method in the medium containing gaseous phase of formiate.;13 cl, 14 dwg, 7 tbl, 4 ex