MAGNETIC-ASSISTED RAPID APTAMER SELECTION METHOD FOR GENERATING HIGH-AFFINITY DNA APTAMER

摘要

PROBLEM TO BE SOLVED: To provide a magnetic-assisted rapid aptamer selection (MARAS) protocol for screening a DNA aptamer.SOLUTION: The MARAS protocol can produce efficiently an aptamer having a high affinity and specificity. In order to select the DNA aptamer having the desired affinity and specificity to a target, a rotating magnetic field or an exchange magnetic field is used in combination with target binding magnetic microparticles or nanoparticles.