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Detection method of human cytomegalovirus infection .

机译:人巨细胞病毒感染的检测方法。

摘要

PROBLEM TO BE SOLVED: To provide new means having a high practical utility capable of detecting HCMV infection at a high sensitivity.;SOLUTION: As a result of an examination of reactivity with serum of a patient with HCMV infection by synthesizing a full-length protein about 15 kinds of HCMV proteins, it is found that all the infection patients can be detected without omission when using a pp28 full-length protein as an antigen. The pp28 full-length protein can be synthesized and refined in large amounts as a recombinant protein by using the Escherichia coli, and can be used commercially as an antigen for detecting HCMV.;COPYRIGHT: (C)2012,JPO&INPIT
机译:解决的问题:提供一种具有高实用性的新方法,能够以高灵敏度检测HCMV感染。;解决方案:通过合成全长蛋白检查HCMV感染患者与血清的反应性大约有15种HCMV蛋白,发现使用pp28全长蛋白作为抗原时,所有感染患者都可以被检出而无遗漏。 pp28全长蛋白可以通过使用大肠杆菌作为重组蛋白进行大量合成和精制,并且可以作为商业抗原用于检测HCMV 。;版权所有:(C)2012,JPO&INPIT

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