Triglyceride synthesis pathway, assays based on cell

摘要

Acyl-CoA: in the absence or presence of a compound, a step of contacting the cell with a stable isotope-labeled fatty acids, methods of identifying a compound that modulates the activity of diacylglycerol acyltransferase, extracting the cells with isopropyl alcohol When, in the presence or absence of compound, and a, and measuring the level of stable isotope-labeled triglyceride, change in the level of stable isotope-labeled triglyceride, indicates that the compound modulates DGAT activity there.