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CELL BASED ASSAYS FOR THE TRIGLYCERIDE SYNTHESIS PATHWAY
CELL BASED ASSAYS FOR THE TRIGLYCERIDE SYNTHESIS PATHWAY
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机译:甘油三酸酯合成途径的基于细胞的分析
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摘要
A method for identifying a compound which modulates the activity of acyl-coA: diacylglycerol acyltransferase comprises the steps of contacting a stable isotope labeled fatty acid with cells in either presence or absence of the compound, extracting the cells with isopropyl alcohol, and determining the level of a stable isotope labeled triglyceride in the presence or absence of the compound; wherein a change in the level of the stable isotope labeled triglyceride indicates that the compound modulates the DGAT activity.
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