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Microarray for detecting specific ticks and method for detecting specific ticks

机译:用于检测特定壁虱的微阵列和检测特定壁虱的方法

摘要

PPROBLEM TO BE SOLVED: To provide a method for specifically detecting the mites, Chelacaropsis moorei and Ornithonyssus bacoti. PSOLUTION: At least one of probe selected from the group consisting of a probe which has a specific base sequence and a probe which has a complementary sequence to the specific base sequence is immobilized on a microarray for detecting specific mites. A certain region of the genomic DNA of mites, Chelacaropsis moorei and/or Ornithonyssus bacoti, contained in a sample is amplified by the PCR method, using the PCR reaction solution which contains a PCR primer pair consisting of a specific base sequence and another specific base sequence, the sample genomic DNA, an enzyme for nucleic acid synthesis, and a substrate for nucleic acid synthesis. Both mites are detected by contacting and binding the amplified DNA to the immobilized probe on the microarray. PCOPYRIGHT: (C)2011,JPO&INPIT
机译:

要解决的问题:提供一种特异性检测螨虫,雪松鼠疫菌和细菌白喉菌的方法。

解决方案:选自具有特定碱基序列的探针和具有与特定碱基序列互补的序列的探针中的至少一种探针固定在用于检测特定螨虫的微阵列上。通过PCR方法,使用包含由特定碱基序列和另一个特定碱基组成的PCR引物对的PCR反应溶液,通过PCR方法扩增样品中所含的螨虫,毛白纹病菌和/或芽孢杆菌基因组DNA的特定区域。序列,样品基因组DNA,用于核酸合成的酶和用于核酸合成的底物。通过将扩增的DNA与微阵列上的固定探针接触并结合,可以检测到两种螨。

版权:(C)2011,日本特许厅&INPIT

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